SubtiBank SubtiBank
Version comparison:

2018-02-23 18:29:052025-05-10 23:09:41

description

trigger enzyme: mannitol-specific phosphotransferase system, EIICB of the PTS

mannitol-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIICB of the [[category|Phosphotransferase system|PTS]], [[category|Trigger enzyme]], control of [[protein|MtlR]] activity

locus

BSU03981

BSU_03981

geneLength

1434

1437

function

mannitol uptake and phosphorylation, control of MtlR activity

mannitol uptake and phosphorylation, control of [[protein|MtlR]] activity

product

trigger enzyme: mannitol-specific phosphotransferase system, EIICB

mannitol-specific [[category|Phosphotransferase system|PTS]], EIICB

outlinks

bsu

BSU03981

BSU_03981

Gene

Coordinates

449,724 → 451,160

449,724 451,160

The protein

Catalyzed reaction/ biological activity

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

D-mannitol + Nπ-phospho-L-histidyl-[protein] --> D-mannitol 1-phosphate + L-histidyl-[protein] (according to UniProt)

The protein

Protein family

[[protein|PtsI]] permease, fructose/ mannitol permease (Fru) family [Pubmed|10627040]

[[category|Phosphotransferase system|PTS]] permease, fructose/ mannitol family [Pubmed|10627040]

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot)

cell membrane (according to UniProt)

Biological materials

Mutant

MGNA-C018 (mtlA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2016 NBRP B. subtilis, Japan]

BKE03981 (Δ[[gene|mtlA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT

BKK03981 (Δ[[gene|mtlA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT

MGNA-C018 (mtlA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2016 NBRP B. subtilis, Japan]

BKE03981 ([[gene|mtlA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT

BKK03981 ([[gene|mtlA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

2 [SW|PTS EIIC domain]s type-2 (aa 18-347, aa 390-478) (according to UniProt)