2018-02-23 18:29:052025-05-10 23:09:41
description
trigger enzyme: mannitol-specific phosphotransferase system, EIICB of the PTS
mannitol-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIICB of the [[category|Phosphotransferase system|PTS]], [[category|Trigger enzyme]], control of [[protein|MtlR]] activity
locus
BSU03981
BSU_03981
geneLength
1434
1437
function
mannitol uptake and phosphorylation, control of MtlR activity
mannitol uptake and phosphorylation, control of [[protein|MtlR]] activity
product
trigger enzyme: mannitol-specific phosphotransferase system, EIICB
mannitol-specific [[category|Phosphotransferase system|PTS]], EIICB
outlinks
bsu
BSU03981
BSU_03981
Gene
Coordinates
449,724 → 451,160
449,724 451,160
The protein
Catalyzed reaction/ biological activity
Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
D-mannitol + Nπ-phospho-L-histidyl-[protein] --> D-mannitol 1-phosphate + L-histidyl-[protein] (according to UniProt)
The protein
Protein family
[[protein|PtsI]] permease, fructose/ mannitol permease (Fru) family [Pubmed|10627040]
[[category|Phosphotransferase system|PTS]] permease, fructose/ mannitol family [Pubmed|10627040]
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane (according to UniProt)
Biological materials
Mutant
MGNA-C018 (mtlA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2016 NBRP B. subtilis, Japan]
BKE03981 (Δ[[gene|mtlA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT
BKK03981 (Δ[[gene|mtlA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT
MGNA-C018 (mtlA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2016 NBRP B. subtilis, Japan]
BKE03981 ([[gene|mtlA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT
BKK03981 ([[gene|mtlA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03981 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT, downstream forward: _UP4_TAATCTTATAGAAAGAGAGT
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
2 [SW|PTS EIIC domain]s type-2 (aa 18-347, aa 390-478) (according to UniProt)